Figure 1.

MoSwi6 interacts with MoMps1. (A) A yeast two‐hybrid assay was used to examine the interaction between MoSwi6 as a bait and MoMps1 as a prey. The interaction between pGBKT7‐53 and pGADT7‐T was used as the positive control, and noninteractions between pGBKT7‐Lam and pGADT7‐T, BD (pGBKT7)‐MoSwi6 and AD (pGADT7), BD and AD‐MoMps1, and empty AD and BD vectors were used as negative controls. Plates were incubated at 30 °C for 3 days before being photographed. (B) Co‐immunoprecipitation assay for the interaction of MoSwi6 with MoMps1. Western blot analysis with total proteins (Total) isolated from transformants co‐expressing the MoSWI6‐GFP and MoMPS1‐3xFLAG constructs and proteins eluted from the anti‐FLAG M2 beads (Elution). The presence of MoSwi6 and MoMps1 was detected with an anti‐GFP and an anti‐FLAG antibody, respectively. GFP, green fluorescent protein.