FIG. 5.

Lung mRNA expression after 4-OPA exposure measured by quantitative real-time PCR. Quantitative real-time PCR analysis of CCL2 (A) CCL19 (B) or IFN-γ (C) mRNA expression. Mice received 4 days of dermal exposure (concentration indicated on graph) followed by a pulmonary challenge of 4-OPA (1%). Bars represent means ± SE of 7 mice per group. Levels of statistical significance are denoted as *p ≤ 0.05 and **p ≤ 0.01 as compared with vehicle control (no dermal or pulmonary exposure to 4-OPA).