FIG. 6.

4-OPA sensitization results in increased cytokine production in the DLN. Analysis of INF-γ (Α), IL-5 (B), and IL-4 (C) protein expression generated by stimulated LALN after long-term exposure to 4-OPA. Mice received 4 days of dermal exposure (concentration indicated on graph) followed by four pulmonary challenges of 4-OPA (1%). Aspiration controls (no dermal exposure) and dermal control (no aspiration exposure) were also included on the graphs. Bars represent mean fold change ± SE of 7 mice per group. Levels of statistical significance are denoted as *p < 0.05 as compared with vehicle control (no dermal or pulmonary exposure to 4-OPA).