Figure 3.

Downregulation of HDAC6 in 5-HT neurons with imipramine and resilience. a, Pictures taken before and after laser capture microdissection of 5-HT neurons from a 10-μm-thick coronal slice of the dorsal raphe nucleus in ePet-YFP transgenic mice. The white triangle indicates the position of the cerebral aqueduct. b, HDAC6 mRNA levels measured by qPCR. Note the absence of regulation of HDAC6 in vulnerable animals receiving vehicle (VUL/VEH), and the significant downregulation of HDAC6 in resilient mice treated with vehicle (RES/VEH) as well as in vulnerable mice treated with 20 mg/kg imipramine (VUL/IMI) (n = 6–8 per condition; **p < 0.01 vs CONT/VEH). Error bars indicate SEM.