Table 1.
Effect of HDAC6 depletion on the distribution, morphology, serotonin synthesis/metabolism, and intrinsic electrophysiological properties of serotonergic neurons: cell body/axon density
| DR somas (nb) | MR somas (nb) | DR TPH (WB) | MR TPH (WB) | DG fibers (% area) | pfCTX fibers (% area) | NAc fibers (% area) | |
|---|---|---|---|---|---|---|---|
| WT | 3856 ± 412.6 | 1433 ± 134.2 | 100.0 ± 21.31 | 100.0 ± 11.21 | 15.26 ± 0.70 | 7.03 ± 0.50 | 16.21 ± 0.12 |
| KO | 3692 ± 601.6 | 1527 ± 201.6 | 127.8 ± 10.8 | 102.39 ± 6.8 | 11.04 ± 2.13 | 6.58 ± 0.90 | 15.33 ± 0.42 |
Lack of difference between behaviorally naïve WT and HDAC6Pet1cre KO mice in number of 5-HT-immunopositive somas in DR and median raphe (MR) nuclei, and the density of 5-HT-immunopositive axons in dentate gyrus (DG), prefrontal cortex (pfCTX), and nucleus accumbens (NAc) estimated stereologically using an optical fractionator and area fraction fractionator probes, respectively (Stereo Investigator software; MBF Bioscience; n = 3 mice/condition). Lack of difference in TPH protein levels measured by Western blot in DR and MR (n = 5–6 per condition).