Table 2.
Effect of HDAC6 depletion on the distribution, morphology, serotonin synthesis/metabolism, and intrinsic electrophysiological properties of serotonergic neurons: somatodendritic morphology
| Soma |
Dendrites |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Volume (μm3) | Surface area (μm2) | Mean length (μm) | Mean area (μm2) | Longest dendrite (μm) | Trees (nb) | Nodes (nb) | Ends (nb) | Mean dendrite length (μm) | |
| WT | 3148.4 ± 653.2 | 954.8 ± 137.3 | 56.5 ± 2.8 | 212.9 ± 19.8 | 321.5 ± 47.5 | 3.7 ± 0.5 | 4.0 ± 0.6 | 272.6 ± 36.4 | 272.6 ± 36.4 |
| KO | 3076.1 ± 672.9 | 974.7 ± 134 | 59.6 ± 2.8 | 229.3 ± 19.4 | 413.1 ± 64 | 4.1 ± 0.3 | 3.4 ± 0.8 | 324.6 ± 42.2 | 324.6 ± 42.2 |
Lack of difference in the detailed morphological characteristics of soma and processes in biocytin-filled 5-HT DR neurons from behaviorally naive WT and HDAC6Pet1cre KO mice (n = 9–12 cells, 1 cell/mouse). All cells were collected from the ventromedial portion of the DR confirmed for serotonergic identity and HDAC6 KO using IHC.