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. 2012 May 1;4(3):373–384. doi: 10.4161/mabs.19581

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graphic file with name mabs-4-373-g7.jpg — Figure 7. Stability analysis of antibodies following incubation at 37°C. (A) Stability of binding capability: ELISA analysis following incubation of the antibodies at 37°C for 4, 8 or 12 d. Binding to ErbB2 of di(H)SFGFP, tetraSFGFP and tetra-tanSF in relation to parental Inclonal FRP5 IgG was evaluated using HRP-conjugated secondary antibodies. The results are presented as the percentage of the signal obtained for 0 d- incubation of each antibody, which was normalized to be 100%. (B) Stability of fluorescence intensity: Fluorimeter analysis following incubation at 37°C for 4, 8 or 12 d. Fluorescence (485-nm excitation and 528-nm emission) of di(H)SFGFP, tetraSFGFP and tetra-tanSF was measured. The results are presented as the percentage of the fluorescence obtained for 0 d- incubation of each antibody, which was normalized to be 100%. Error bars represent standard deviations of triplicates.