Table 1. Retention factor k’ and pI of murine mAb derived from hybridoma1.
|
mAb # |
k'
2
|
k' after deglycosylation |
pI |
| 1 |
0.01 ± 0.02 |
|
7.2 |
| 2 |
0.00 |
0.03 |
7.2 |
| 3 |
-0.02 ± 0.04 |
0.00 |
N.D. |
| 4 |
0.02 |
|
7.1 |
| 5 |
0.01 ± 0.01 |
|
7.1 |
| 6 |
0.00 |
|
7.3 |
|
7 |
0.02 |
|
N.D. |
|
8 |
0.00 |
|
N.D. |
| 9 |
0.00 ± 0.00 |
|
7.7 |
| 10 |
0.00 |
|
7.3 |
| 11 |
0.01 ± 0.03 |
|
N.D. |
| 12 |
0.01 ± 0.01 |
|
7.2 |
| 13 |
0.02 ± 0.04 |
|
5.9 |
| 14 |
0.01 ± 0.02 |
|
7.0 |
| 15 |
0.01 ± 0.01 |
|
N.D. |
| 16 |
0.00 |
|
N.D. |
| 17 |
0.03 ± 0.01 |
|
8.3 |
| 18 |
0.18 |
|
6.5 |
| 19 |
-0.01 ± 0.06 |
|
N.D. |
|
20 |
0.04 |
|
N.D. |
| 21 |
0.01 ± 0.01 |
|
7.2 |
| 22 |
0.11 ± 0.15 |
0.05 |
7.2 |
| 23 |
0.03 ± 0.01 |
|
7.3 |
| 24 |
0.09 |
|
7.0 |
| 25 |
0.02 ± 0.01 |
|
7.4 |
| 26 |
0.16 |
0.13 |
N.D. |
| 27 |
0.01 ± 0.00 |
|
7.0 |
|
28 |
0.09 |
|
N.D. |
| 29 |
0.13 |
0.15 |
6.9 |
| 30 |
0.00 |
|
N.D. |
|
31 |
0.00 |
|
N.D. |
|
32 |
0.00 |
|
N.D. |
| 33 |
0.05 ± 0.01 |
0.06 |
6.9 |
|
34 |
0.09 |
|
N.D. |
| 35 |
0.04 |
0.05 |
N.D. |
| 36 |
0.01 ± 0.03 |
|
7.2 |
| 37 |
0.00 |
|
8.0 |
| 38 |
0.07 |
|
N.D. |
|
39 |
0.00 ± 0.00 |
|
N.D. |
| 40 |
0.02 ± 0.01 |
|
6.9 |
| 41 |
0.04 |
|
N.D. |
| 42 |
0.06 ± 0.03 |
|
7.0 |
| 43 |
0.00 ± 0.03 |
|
N.D. |
| 44 |
0.05 |
|
N.D. |
|
45 |
0.00 |
|
7.4 |
| 46 |
0.00 ± 0.00 |
|
7.4 |
| 47 |
0.12 ± 0.02 |
|
7.7 |
| 48 |
0.00 |
|
7.2 |
| 49 |
0.09 ± 0.01 |
|
7.3 |
|
50 |
0.04 |
|
N.D. |
| 51 |
0.04 ± 0.01 |
|
7.6 |
| 52 |
0.04 ± 0.00 |
|
6.9 |
| 53 |
0.03 ± 0.01 |
0.00 |
N.D. |
| 54 |
0.16 |
|
N.D. |
| 55 |
0.00 ± 0.04 |
|
7.1 |
| 56 |
0.04 |
|
N.D. |
| 57 |
0.04 ± 0.01 |
|
7.4 |
| 58 |
0.03 |
|
N.D. |
|
59 |
0.01 |
|
7.3 |
| 60 |
0.01 ± 0.01 |
|
N.D. |
| 61 |
0.06 ± 0.01 |
|
N.D. |
| 62 |
0.06 ± 0.01 |
|
7.6 |
|
63 |
0.07 ± 0.10 |
|
7.6 |
|
64 |
0.06 |
|
N.D. |
| 65 |
0.00 |
|
7.7 |
| 66 |
0.04 ± 0.06 |
|
6.9 |
| 67 |
0.03 ± 0.04 |
0.11 |
5.8 |
| 68 |
0.02 ± 0.03 |
|
7.0 |
|
69 |
0.04 |
|
N.D. |
|
70 |
0.08 |
|
N.D. |
| 71 |
0.02 ± 0.03 |
0.05 |
7.8 |
|
72 |
0.12 |
|
N.D. |
| 73 |
0.03 |
|
N.D. |
|
74 |
0.03 |
|
N.D. |
| 75 |
0.01 |
|
7.0 |
| 76 |
0.03 ± 0.04 |
|
8.3 |
|
77 |
0.07 |
|
N.D. |
|
78 |
0.13 |
|
N.D. |
|
79 |
0.03 |
|
7.2 |
| 80 |
0.04 |
|
6.9 |
|
81 |
0.07 |
|
N.D. |
| 82 |
0.02 ± 0.02 |
|
N.D. |
|
83 |
0.05 |
|
N.D. |
|
84 |
0.04 |
|
N.D. |
| 85 |
0.03 |
|
6.6 |
|
86 |
0.04 |
|
N.D. |
| 87 |
0.05 ± 0.04 |
0.00 |
N.D. |
|
88 |
-0.02 |
|
N.D. |
|
89 |
0.09 |
|
6.9 |
|
90 |
0.01 |
|
5.5 |
|
91 |
-0.01 |
|
6.2 |
| 92 | -0.03 | 5.5 |
1Samples with italic numbers were not subject to MALDI-TOF-MS analysis; Samples 89–92 have V-region sequences available and do not contain consensus N-linked glycosylation site; for the antibodies identified to contain V-region glycosylation, the retention factors (k’) upon deglycosylation are listed. 2The errors are calculated from n = 2 chromatographic experiments