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Figure 1. (A) Schematic representation of an IgG1 antibody showing sites of conjugation (thiol, amine and carbohydrate) used in generating the antibody-biotin conjugates. (B) Structure and length of linkers used in conjugating biotin (biocytin) to antibody. N-hydroxysuccinimide (blue), spacer arm (black), maleimide (violet), biotin (biocytin) (green).
