Figure 2. Antigen binding activity of select AbX AF-blends measured in a cell-based binding assay. Human B lymphoma cell line WIL2-S were coated on plates and incubated with various concentrations of AbX AF-blends. Antibodies bound to CD20 on WIL2-S were detected with goat anti-human IgG polyclonal antibodies. Results for AbX AF-blends containing 0% (○), 5% (□), 10% (△), 20% (◇), 50% (●), and 100% (■) of the afucosylated material are shown. RLU = Relative luminescence unit