Figure 4. Binding responses of AbX AF-blends to human Fcγ receptors. Dose-response binding curves of AbX AF-blends to various human Fcγ receptors were generated from ELISA-based binding assays using soluble recombinant Fcγ receptors. The test antibodies were assayed in monomeric form for (A) Fcγ Receptor Ia, and in multimeric forms for (B)IIa-R131, (C)IIb, (D)IIIa-F158, (E)IIIa-V158. All data points were collected in duplicate and the mean absorbance values from duplicate wells at OD 450nm were plotted against the antibody concentration. Binding curves for 0% (blue ○), 2% (green □), 5% (orange △), 7.5% (magenta ◇), 10% (gray ●), 20% (brown ■), 50% (purple ▲), 100% (red ◆) of the afucosylated material are shown. The results shown were obtained from one representative data set out of at least three in vitro binding experiments.