Table 1. Levels of afucosylated glycans in AbX AF-blends.
|
Afucosylated Material |
Theoretical G0-F (T) |
Observed |
Difference |
|
G0-F (O) |
(O-T)/T |
||
| 100% |
100% |
98% |
-2.2% |
| 50% |
51% |
46% |
-8.6% |
| 20% |
21% |
18% |
-15% |
| 17.5% |
19% |
16% |
-16% |
| 15% |
16% |
14% |
-16% |
| 12.5% |
14% |
11% |
-16% |
| 10% |
11% |
9.5% |
-15% |
| 7.5% |
8.7% |
7.4% |
-15% |
| 5% |
6.2% |
5.4% |
-13% |
| 4% |
5.2% |
4.8% |
-8.2% |
| 3% |
4.3% |
4.1% |
-4.0% |
| 2% |
3.3% |
3.2% |
-3.8% |
| 1% |
2.3% |
2.3% |
0.1% |
| 0% | 0% | 1.3% | 1.3% |
Theoretical G0-F was calculated assuming that the “regular” fucosylated material contains 1.3% afucosylated glycans and the afucosylated material contains 100% of afucosylated glycans; for example, the theoretical G0-F value of AbX AF-blend containing 10% afucosylated material is calculated as 0.1 x100% + 0.9 x 1.3% = 11%. Observed G0-F values are obtained from the CE-based glycan analysis.