Table 4.
Observed point mutants.
| Mutant | CesA | Species | Mutation | Region | CesA conserved | CslD conserved | CslF conserved | Reference |
|---|---|---|---|---|---|---|---|---|
| rsw1-1 | 1 | A. thaliana | A549 → V | Catalytic | A or G | A | G | Baskin et al. (1992) |
| rsw1-2 | 1 | A. thaliana | G631 → S | Catalytic | G | G | G | Gillmor et al. (2002) |
| rsw1-45 | 1 | A. thaliana | E779 → K | Catalytic | E | E | E | Beeckman et al. (2002) |
| rsw1-20 | 1 | A. thaliana | D780 → N | Catalytic | D | D | D | Beeckman et al. (2002) |
| eli1-1 | 3 | A. thaliana | S301 → F | 2nd TM | S | S | mostly S; also M, T |
Cano-Delgado et al. (2000) |
| eli1-2 | 3 | A. thaliana | A522 → V | Catalytic | A | A | A | Cano-Delgado et al. (2000) |
| tha1** | 3 | A. thaliana | P578 → S | Catalytic | P | P | P | Daras et al. (2009) |
| cev1 | 3 | A. thaliana | G617 → E | Catalytic | G | G | G | Ellis et al. (2002) |
| ixr1-2* | 3 | A. thaliana | T942 → I | 3rd Outer | T | T | T | Scheible et al. (2001) |
| ixr1-1* | 3 | A. thaliana | G998 → D | 7th TM | G | G | G,M,L,A | Scheible et al. (2001) |
| rsw5 | 3 | A. thaliana | P1056 → S | C-terminus | P except in CesAs 1, 8 |
Absent | Absent | Wang et al. (2006) |
| bc11 | 4 | O. sativa | G858 → R | 5th TM | G | G | mostly G; also K, R, L |
Zhang et al. (2009) |
| ixr2-1* | 6 | A. thaliana | R1064 → W | C-terminus | R, K | Absent | Absent | Desprez et al. (2002) |
| irx1-1 | 7 | A. thaliana | G683 → N | Catalytic | G | N | Absent | Taylor et al. (2000) |
| fra5** | 7 | A. thaliana | P557 → T | Catalytic | P | P | P | Zhong et al. (2003) |
| mur10-2 | 7 | A. thaliana | H805 → Y | Catalytic | H | H | H | Bosca et al. (2006) |
| irx1-2 | 8 | A. thaliana | S679 → L | Catalytic | S | S | T, I | Taylor et al. (2000) |
| lew2-2 | 8 | A. thaliana | L791 → F | 2nd outer | L | L | P | Chen et al. (2005) |
A list of all missense mutations observed in the CesA family which have been categorized. Region indicates which part of the gene the mutation occurs in (TM is transmembrane domain, catalytic refers to the second cytoplasmic loop). Also listed are the conserved amino acids in the global alignment at the position of the mutation. Additionally, the dominant amino acids in the CslDs and CslFs at the same position are reported.
*These mutations confer resistance to the drug isoxaben but do not impair CesA function.
**These mutations are dominant-negative (and occur at the same position in the alignment).