Table 1.
Polar lipid molecular species significantly changed at end of dark or light period.
| Lipid | Fatty acyl species | Time that the indicated lipid is highest (see legend for details) |
|---|---|---|
| Total analyzed lipids | dark/not applicable | |
| PA 34:2 | 18:2–16:0 | Dark/– |
| PA 34:3 | 18:3–16:0 > 18:2–16:1 | Dark/dark |
| PA 36:4 | 18:2–18:2 > 18:3–18:1 | Dark/– |
| PA 36:5 | 18:2–18:3 | Dark/dark |
| PA 36:6 | 18:3–18:3 | Dark/(dark) |
| Total PA | Dark/(dark) | |
| PS 34:2 | Dark/(dark) | |
| PS 34:3 | Dark/dark | |
| PS 36:2 | 18:2–18:0 | (Dark)/– |
| PS 36:3 | 18:3–18:0, 18:2–18:1 | (Dark)/– |
| PS 36:4 | 18:3–18:1, 18:2–18:2 | (Dark)/– |
| PS 36:5 | 18:3–18:2 | Dark/dark |
| PS 38:2 | (Dark)/– | |
| PS 38:3 | (Dark)/– | |
| PS 38:4 | (Dark)/– | |
| PS 40:2 | 22:0–18:2 | Dark/(dark) |
| PS 40:3 | 22:0–18:3 | Dark/– |
| PS 42:2 | 24:0–18:2 | Dark/dark |
| PS 42:3 | 24:0–18:3, 24:1–18:2 | Dark/dark |
| PS 42:4 | 24:1–18:3 | Dark/dark |
| PS 44:3 | 26:0–18:3 | (Dark)/(dark) |
| Total PS | Dark/dark | |
| PI 34:1 | 16:0–18:1 | (Light)/(light) |
| PI 34:2 | 16:0–18:2 | (Dark)/– |
| PI 34:3 | 18:3–16:0 | (Dark)/– |
| PI 36:3 | 18:1–18:2, 18:0–18:3 | Light/light |
| PI 36:4 | 18:2–18:2, 18:1–18:3 | Light/light |
| PI 36:6 | 18:3–18:3 | (Dark)/– |
| Total PI | (Dark)/– | |
| PE 36:3 | 18:0–18:3, 18:1–18:2 | –/Light |
| PE 38:2 | 20:0–18:2 | –/(Light) |
| PC 32:0 | (Dark)/dark | |
| PC 34:1 | 16:0–18:1 | Light/light |
| PC 34:2 | 16:0–18:2 | (Dark)/– |
| PC 34:3 | 16:0–18:3 | Dark/(dark) |
| PC 34:4 | 16:1–18:3 | Dark/(dark) |
| PC 36:2 | 18:0–18:2 > 18:1–18:1 | Light/light |
| PC 36:3 | 18:1–18:2 > 18:0–18:3 | Light/light |
| PC 36:4 | 18:2–18:2 > 18:1–18:3 | (Light)/light |
| PC 36:5 | 18:3–18:2 | Dark/(dark) |
| PC 36:6 | 18:3–18:3 | Dark/dark |
| PC 38:2 | –/Light | |
| PC 38:3 | 20:1–18:2 > 20:0–18:3 | –/Light |
| PC 38:6 | Dark/– | |
| PC 40:4 | (Dark)/– | |
| PC 40:5 | (Dark)/– | |
| Total PC | (Dark)/– | |
| PG 32:0 | 16:0–16:0 | (Dark)/– |
| PG 32:1 | 16:0–16:1 | Dark/dark |
| PG 34:1 | 18:1–16:0 > 16:1–18:0 | Light/light |
| PG 34:2 | 18:2–16:0, 16:1–18:1 | (Dark)/– |
| PG 34:3 | 18:3–16:0 > 16:1–18:2 | Dark/(dark) |
| PG 34:4 | 18:3–16:1 | Dark/– |
| Total PG | Dark/– | |
| MGDG 34:1 | 18:0–16:1 > 16:0–18:1 | (Light)/light |
| MGDG 34:2 | 18:1–16:1 > 16:0–18:2 | Light/light |
| MGDG 34:3 | 16:0–18:3, 18:2–16:1, 18:0–16:3, 18:1–16:2 | Light/light |
| MGDG 34:4 | 18:3–16:1, 18:2–16:2, 18:1–16:3 | Light/light |
| MGDG 34:5 | 18:3–16:2, 18:2–16:3 | –/(Light) |
| MGDG 34:6 | 18:3–16:3 | Dark/(dark) |
| MGDG 36:2 | (Light)/light | |
| MGDG 36:3 | 18:3–18:0 | (Light)/(light) |
| MGDG 36:6 | 18:3–18:3 | Dark/(dark) |
| Total MGDG | Dark/– | |
| DGDG 34:1 | –/Light | |
| DGDG 34:3 | 16:0–18:3 > 18:2–16:1 | Dark/– |
| DGDG 34:4 | Dark/– | |
| DGDG 34:5 | 18:3–16:2 > 18:2–16:3 | (Dark)/– |
| DGDG 34:6 | 18:3–16:3 | Dark/– |
| DGDG 36:3 | 18:3–18:0, 18:2–18:1 | (Dark)/– |
| DGDG 36:4 | 18:2–18:2, 18:1–18:3 | (Dark)/– |
| DGDG 36:6 | 18:3–18:3 | Dark/(dark) |
| DGDG 38:4 | (Dark)/– | |
| DGDG 38:6 | 20:3–18:3 | Dark/dark |
| Total DGDG | Dark/– | |
| LPC 18:1 | Light/light | |
| LPC 18:2 | –/(Light) | |
| LPG 18:1 | (Light)/light |
Forty- to 46-day-old Arabidopsis thaliana plants, grown at 21°C with a 12-h light/12-h dark cycle were sampled 11 h into the dark and light periods for four dark–light cycles. The experiment was repeated three times. For each experiment, data (five biological replicates at each sampling point) from the dark periods were pooled, and data from the light periods were pooled. Data from all three experiments showing a significant difference (p < 0.05) in the same direction between dark and light periods are indicated as “dark” or “light”; changes in two experiments that were significant, with the third showing a change in the same direction, are indicated as “(dark)” or “(light).” The significant effects shown in the third column are based on normalized lipid MS signal per extracted dry mass (listed first) and percentage of normalized lipid MS signal (after slash); a hyphen indicates no significance. Complete details are in Tables S1 and S2 in Supplementary Material. Fatty acyl species in each class were determined previously (Welti et al., 2002; Devaiah et al., 2006; Burgos et al., 2011; Samarakoon et al., 2012) and are summarized in Samarakoon et al. (2012).