Fig. 7.
Knockdown of THRB rescues TRH despite concurrent T3 treatment. TαT1.1 cells were treated as indicated in the upper part of the figure. Tshb relative mRNA after vehicle (white bars) or TRH 100 nm (gray bars) is compared. The x-axis shows the different shRNA used. Data were normalized for each mRNA relative to the samples without T3 or TRH treatment and exposed to scrambled shRNA viral infection. Data are shown as mean ± sem of at least three separate experiments performed in triplicate. *, P < 0.05; **, P < 0.01.