Fig. 3.

JNK1 and ERα colocalize at promoters of “JNK1-recruited” genes. A and B, ChIP-qPCR (top) and reChIP-qPCR (bottom) were performed reciprocally for ERα and JNK1 for three JNK1-recruited gene promoters (GREB1, CYP1B1, and PCYT1A) and one “JNK1-consititutive” gene promoter (ACO2) before (U) and after (E) E2 treatment. The initial ChIP (top) was performed using antibodies to ERα (A) or JNK1 (B). The recovered ChIP DNA was then immunoprecipitated using antibodies to JNK1 (A) or ERα (B) in a reChIP experiment (bottom). The ChIP and reChIP DNAs were analyzed by RT-qPCR. Each bar represents the mean ± sem, n = 3.