Fig. 7.

JNK1 is required for full estrogen-dependent growth responses in MCF-7 cells. A, Analysis of E2-dependent cell growth in control (GFP) and JNK1 knockdown (KD) MCF-7 cells grown for 10 d in the absence (U) or presence (E) of E2. Two independent JNK1 shRNA (KD1 and KD2) constructs were used, as shown, and the number of cells in each condition was counted every 2 d. Each point represents the mean ± sem, n = 3. Statistical analysis was performed by ANOVA for each time point (P < 0.05). Groups a–c are not statistically different from each other at d 0, 2, and 4. Groups b and c are statistically different from group a, but not each other, at d 6. Groups a–c are statistically different from each other at d 8 and 10. B, JNK1 expression is elevated in breast carcinomas. Data obtained from gene expression analyses were analyzed using Oncomine. The relative expression of MAPK8 (i.e. JNK1) from four normal breast stroma samples and 51 breast tumor samples is shown. The Oncomine-reported P value was less than 3.0 × 10⁻⁴. The values were normalized to an average expression level of 1 for the normal breast samples. Red lines represent the average signal in each category.