Cold tolerance, plasma inflammatory markers, plasma triglycerides, and glucose handling in Tg(ENPP2) mice. A, Body temperature of Tg(ENPP2) and WT mice (n = 4) was measured at room temperature and at different intervals up to 6 h at 4 C. Values are means ± sd. B, Circulating inflammatory markers, IL6, TNFα, insulin, leptin, and tPAI were measured in plasma from Tg(ENPP2) and WT mice (n = 10) on a Bio-plex 200 Suspension array System (Bio-Rad) using Mouse Serum Adipokine LINCOplex Kit. Normalized fluorescent unit (FU) values are reported. C, Intraperitoneal glucose tolerance test was performed by ip injection of glucose (2 g/kg body weight in isotonic saline) (n = 4 per group). D, Plasma triglycerides were measure by Wako L-Type TG M microtiter plate assay kit (n = 10). Values are means ± sd.