Fig. 6.

CnAβ-deficient mice have reduced intra-acinar zymogen activation and cell injury induced by caerulein. Acinar cells from WT or CnAβ−/− mice were treated with caerulein (0.01–100 nM) for 30 min, and trypsin (A) and chymotrypsin (B) activities were normalized to total amylase content (n = 3). #*P < 0.05 with respect to the unstimulated condition or WT acinar cells, respectively. C: acinar cells were treated with 100 nM caerulein for 2 h, and cell injury was measured as percent LDH released (n = 2). #P < 0.001, with respect to WT acinar cells receiving 100 nM caerulein. D: biphasic amylase secretion curve (n = 3). Data are means ± SE.