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. 2012 May 4;3:80. doi: 10.3389/fpls.2012.00080

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Copyright © 2012 Funck, Clauß, Frommer and Hellmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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rsr1-1 has reduced chlorophyll and forms spontaneous lesions. (A) Chlorophyll was extracted from 30-day-old soil grown plants. Chlorophyll A and B content was significantly reduced in the rsr1-1 mutant compared to the parental line Pat(B33)-Gus. Values presented are the mean ± SD from 10 plants. (B) Under long-day conditions in the greenhouse, rsr1-1 mutants displayed spontaneous lesion formation on all leaves. (C) Infiltration with 3,3′-diaminobenzidine demonstrated ROS accumulation in lesions of rsr1-1 leaves.