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. 2012 May 4;3:80. doi: 10.3389/fpls.2012.00080

Figure 3.

Figure 3

Sugar insensitivity of germination and establishment in rsr1-1. (A,B) Germination rates of rsr1-1 and Pat(B33)-Gus seeds on MS-medium supplemented with different concentrations of Glc, 3Omeg, or sorbitol. Only on 330 mM Glc and on 30 mM Glc + 300 mM 3Omeg significant differences were observed between rsr1-1 and Pat(B33)-Gus. Values represent the mean ± SD of three replica of 70 seeds each. (C) On plates containing 300 mM Glc both rsr1-1 and Pat(B33)-Gus were able to initiate chlorophyll synthesis and the formation of true leaves. (D) 330 mM D-Glc inhibited establishment of autotrophic growth of Pat(B33)-Gus whereas rsr1-1 could still develop normally. (E) After 2 months on MS-medium supplemented with 360 mM D-Glc, rsr1-1 developed callus-like enlargements of the apical meristem, which were not observed in Pat(B33)-Gus (F).