Table A1.
Markers around RSR1 on chromosome I to distinguish between Col-8 and C24 DNA.
| Marker | Marker type (enzyme) | Length/no. of sites | Primer | Sequence (5′ → 3′) |
|---|---|---|---|---|
| F12A21.4 | CAPS (HindIII) | C24: 1 | F12A21.4-F | CCTATGAGAGTGACGACTCT |
| Col-0: 0 | F12A21.4-Rs | CACGTGTTGACAACTCTCTTTC | ||
| F20P5.1 | CAPS (HincII) | C24: 3 | F20P5.1-F | GCCAGCTTCACTTCCCATTT |
| Col-0: 1 | F20P5.1-Rs | AGTCTCTATGCTCCTAACGAC | ||
| nga111 | SSLP | C24 > Col-0 | nga111-F | See TAIR-DB |
| nga111-R | ||||
| BW54 | CAPS (EcoRV) | C24: 0 | BW54m-F | GATCGGTATACTTAGTTAATTACG |
| Col-0: 1 | BW54m-R | TGTTTGGTGTCCGAGTCACTG | ||
| f6d5 | CAPS (HincII) | C24: 2 | F6D5-F | ACGGGATCCAAAACCACTTG |
| Col-0: 3 | F6D5-R | GTCTGCCTCGTCAGGAGATC | ||
| F25P22.3 | CAPS (ScrFI) | C24: 1 | F25P22.3-F | CTCCAAAAGCAATGACCAATG |
| Col-0: 2 | F25P22.3-R | GTCAATCCCTTTAATCAACGG | ||
| F25P22.7 | CAPS (TaqI) | C24: 7 | F25P22.7-F | TGCTACCTGAACCATACAGAG |
| Col-0: 8 | F25P22.7-R | CACACGAACAAGATAAGGCTG | ||
| F25P22.9 | CAPS (BspHI) | C24: 1 | F25P22.9-F | CCTCTAAATCAATGGGTGGTG |
| Col-0: 2 | F25P22.9-R | AACTCTACTATGGGGTATAGC | ||
| F25P22.10 | CAPS (AccI) | C 24: 2 | F25P22.10-F | TATGGCCTATGATGGCCTATG |
| Col-0: 1 | F25P22.10-R | CATTATTTTGGGGATAGGGATG | ||
| f25p22 | SSLP | Col-0 > C24 | F25P22-F | AAAAACACTTAAAGAGTGAATAG |
| F25P22-R | GAAATGTGATTGAATGTACAAC | |||
| f25p22.68 | CAPS (TaqI) | C24: 2 | F25P22.68-F | TAATCGAACCACCATTGTAGC |
| Col-0: 1 | F25P22.68-R | AACTTAAGAAAGCCCTTTCAC | ||
| f25p22.95 | SSLP | C24 > Col-0 | F25P22.95-F | TCTCCTCCAAAGGTCTCTATC |
| F25P22.95-R | GCTAAAATCCGAAAACAAGGG | |||
| f2p9.46 | CAPS (NdeI) | C24: 2 | F2P9.46-F | TTACCCACCAATGCAGGAACC |
| Col-0: 1 | F2P9.49-R | CTTCACCTAACGGAAGAAGAG | ||
| f1o17.35 | CAPS (XbaI) | C24: 1 | F1O17.35-F | TCACCAGACGAAATCTCAAC |
| Col-0: 2 | F1O17.38-R | ATCCGATGTTTCATTCGTCG | ||
| f1m20 | SSLP | Col-0 > C24 | F1M20-F | CCTTGACCTGTTCCTTCTTTG |
| F1M20-R | CATTCAAAGTACTAATCCGCAC | |||
| f25a4 | SSLP | C24 > Col-0 | F25A4-F | GATTCTCTTCCGACCCTCAC |
| F25A4-R | TTGAACGTCAAAGGACGCAG | |||
| ATPase | SSLP | Col-0 > C24 | ATPase-F | GTTCACAGAGAGACTCATAAACCA |
| ATPase-Rn | AAGTGAAACAACTTGGTACAAGG | |||
| ADH | CAPS (XbaI) | C24: 1 | ADH-F | See TAIR-DB |
| Col-0: 0 | ADH-R |
Markers are listed according to their position on chromosome I with F12A21.4 being most centromeric. Names are derived from the BAC clones which harbor the respective marker, unless previously described markers were used (nga111 and ADH) or modified (BW54 and ATPase). SSLP, simple sequence length polymorphism; CAPS, cleaved amplified polymorphism.