Table 3.
Dilution, blocking, and designated sections for all primary antibodies.
| Primary antibody | Source | Dilution | Blocking | Designated sections |
|---|---|---|---|---|
| Mouse anti-GFAP | Sigma | 1:400 | 4% goat serum (Sigma) + | 7 μm-thick paraffin-embedded sections |
| Rabbit anti-GAD65/67 | Sigma | 1:400 | 2% BSA (Sigma) | |
| Mouse anti-NeuN | Chemicon | 1:100 | 30 μm-thick freezing microtome sections | |
| Rabbit anti-Iba1 | Wako | 1:1000 (+0.3% Triton X-100) | Dyna Antibody Diluant (Dyna Scientific) (+0.5% Triton X-100) |
Abbreviations: GFAP, glial fibrillary acidic protein; GAD, glutamic acid decarboxylase; NeuN, neuronal nuclei; Iba1, ionized calcium binding adaptor molecule 1.