Table 2.

Relative metabolite levels (to the internal standard ribitol) measured by GC–MS of whole rosette leaves harvested after 6 h in the light period from plants grown at 100 μmol quanta m⁻² s⁻¹.

	wild type Mean ± SE	GMK3 Mean ± SE	Fold-changes
Alanine	18.643 ± 0.192	16.936 ± 1.255	0.91
Glutamate	30.813 ± 2.808	31.011 ± 4.928	1.01
Aspartate	10.148 ± 1.107	6.549 ± 1.764	0.74
Threonine	8.584 ± 0.480	11.06 ± 2.173	1.29
Asparagine	5.363 ± 0.576	1.644 ± 0.781	0.30
Valine	2.467 ± 0.130	3.570 ± 0.132	1.45
Proline	1.268 ± 0.596	3.881 ± 2.422	3.06
Alanine	0.434 ± 0.084	0.367 ± 0.095	0.85
Cysteine	0.022 ± 0.014	0.036 ± 0.008	1.64
Tyrosine	0.065 ± 0.014	0.056 ± 0.015	0.86
Methionine	0.602 ± 0.023	0.757 ± 0.168	1.26
Lysine	0.657 ± 0.085	0.736 ± 0.062	1.12
Isoleucine	0.694 ± 0.040	1.207 ± 0.492	1.74
Leucine	0.421 ± 0.053	0.646 ± 0.110	1.53
Phenylalanine	0.610 ± 0.031	1.144 ± 0.206	1.87
Maltose	2.214 ± 1.972	5.382 ± 3.945	2.43
Mannose	0.162 ± 0.021	0.223 ± 0.041	1.38
Lactose	0.074 ± 0.003	0.073 ± 0.005	0.99
Xylose	0.171 ± 0.015	0.197 ± 0.03	1.15
Sorbitol	0.317 ± 0.061	0.231 ± 0.123	0.73
Mannitol	1.837 ± 0.203	1.600 ± 0.406	0.89
Glucose	34.350 ± 6.530	26.862 ± 13	0.78
Myoinositol	31.389 ± 3.792	39.926 ± 7.585	1.27
Fructose	5.915 ± 1.511	8.047 ± 3.023	1.36
Raffinose	6.479 ± 0.534	3.415 ± 0.925	0.53
Glycerol	9.517 ± 0.336	5.614 ± 0.672	0.59
Sucrose	239.223 ± 23.471	204.220 ± 46.941	0.85
Oxalate	0.726 ± 0.070	0.52 ± 0.139	0.72
Succinic acid	1.653 ± 0.519	2.723 ± 1.039	1.65
2-Ketoglutarate	0.054 ± 0.002	0.139 ± 0.004	2.57
Glycerate	2.542 ± 0.368	3.308 ± 0.736	1.30
Gluconate	0.478 ± 0.023	0.326 ± 0.046	0.68
GABA	0.266 ± 0.054	0.233 ± 0.109	0.88
Quinate	0.052 ± 0.011	0.028 ± 0.023	0.54
Glycolate	4.495 ± 0.068	2.708 ± 0.137	0.60
Shikimate	3.353 ± 0.396	3.926 ± 0.792	1.17
d-2-hydroxyglutarate	0.095 ± 0.007	0.171 ± 0.014	1.80
Phosphoric acid	1.319 ± 0.045	0.471 ± 0.091	0.36
Lactate	10.584 ± 0.462	7.479 ± 0.8	0.71
Maleate	0.717 ± 0.019	1.030 ± 0.039	1.43
Malate	14.611 ± 1.386	21.438 ± 2.771	1.47
Fumarate	179.719 ± 8.767	115.055 ± 17.53	0.64
(Iso)-citrate	16.439 ± 1.446	30.208 ± 2.981	1.84

The values are normalized per gram fresh weight and presented are the means ± SE of two replicates of pools of at least eight plants each. The values set in bold case indicate significant differences to the wild type values (Student’s t-test P < 0.05). The data is also expressed as fold-changes of the metabolite levels between GMK3 and wild type.