Figure A2.

Quality control of fragmented mRNA. The poly(A) RNA used for the fragmentation experiments was purified using oligo(dT) at least three times, the quality of the mRNA was confirmed by RNA6000 LabChip (Agilent) (A). After fragmentation the average fragment size was confirmed by RNA6000 LabChip (Agilent) (B). A dot-blot was used for confirming that the fragments after fractionation were enriched in the predicted parts of the mRNA pool. Probes were made from the 5′, the middle and the 3′ regions of the ATPC mRNA and it was hybridized to membrane bound RNA pools of the different fractionated fragments (C).