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. 2011 Jul 18;2:32. doi: 10.3389/fpls.2011.00032

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Copyright © 2011 van Verk, Neeleman, Bol and Linthorst.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Nuclear localization of NtWRKY12 and TGAs. The panels on the middle row show confocal laser scanning microscopy images obtained of Arabidopsis protoplasts transfected with expression plasmids encoding unfused CFP (A) and YFP (B), and fusion proteins NtWRKY12:CFP [(C) W12:CFP], TGA2.1:YFP (D), TGA2.2:YFP (E), and NtNPR1:CFP (F). Panels on the bottom row show overlays with bright-field images (top row) of the same protoplasts. Localizations were visualized with a 63× objective. The red rulers indicate 5 μm.