Figure 4.

Mapping of the functional domains of NtWRKY12. NtWRKY12 peptides fused to the GAL4 DNA-binding domain were tested for their ability to activate GAL4::Ade gene expression in yeast (results with these constructs are indicated in column BD). NtWRKY12 peptides fused to the GAL4 activation domain were tested for their ability to bind to PR-1a promoter fragment controlling the HIS marker gene (results with these constructs are indicated in column AD). Unfused NtWRKY peptides were assayed for binding to the PR-1a promoter and activation of the HIS gene under its control (results with these constructs are indicated in column –). Column aa shows the ranges of the amino acid residues of the respective NtWRKY12 peptides. Plus signs in columns BD, AD, and – indicate growth of yeast; minus signs indicate absence of growth of yeast. The absence of plus or minus signs: not tested.