Figure 5.

Activation of PR-1a::GUS in WT Col-0 and npr1-1mutant lines. Leaf protoplasts were cotransfected with PR-1a::GUS constructs together with expression plasmids containing 35S::NtWRKY12 (W12), 35S::NtNPR1 (NtNPR1), 35S::TGA2.1 (TGA2.1), 35S::TGA2.2 (TGA2.2), a combination, or with empty expression vector, as indicated by the plus and minus signs. (A) Expression in protoplasts isolated from seedlings of WT Col-0 Arabidopsis, (B) expression in npr1-1 protoplasts. The bars represent the percentage of GUS activity from triplicate experiments relative to that of the protoplasts cotransfected with the corresponding PR-1a::GUS construct and empty vector control. Error bars represent the SEM.