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Analysis of high-mannose-containing N-glycans with ConA affinity blotting. (A) Twenty-two micrograms of total soluble protein from red fruits of L. esculentum cv. MicroTom wild type (WT, lane 1), XylT_RNAi line 3 (lanes 2–3), or XylT_RNAi line 12 (lanes 4–5) were loaded per lane. Fruits analysed were derived from the T1-generation of transgenic plants. Lane 2, XylT_RNAi 3–1; lane 3, XylT_RNAi 3–7; lane 4 XylT_RNAi 12–4; lane 5, XylT_RNAi 12–12. (B) Silver staining of protein extracts.
