Figure 7.

PLD-derived PA is specifically involved in the regulation of the actin dynamics. Pollen tube cultures (90 min after imbibition in sucrose medium) were treated with a particular drug, PA or the corresponding volume of control solution for 5 or 20 min before fixation. The actin cytoskeleton was visualized by Alexa Fluor 633 Phalloidin as described in Pleskot et al. (2010). At least 20 cells were analyzed for each treatment in three independent experiments and typical examples are shown. Scale bar = 10 μm.