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RT-PCR analysis of OsDGK transcripts in rice cells treated with xylanase. Suspension cells were treated with 200 μg/ml xylanase for the indicated times, and OsDGK transcripts were monitored by RT-PCR. Approximately 0.1 μg of total RNA was used in each PCR. OsDGK6 was undetectable in cells with or without xylanase treatment. The OsGAPDH gene was used as an internal control.
