Figure 7.

Effects of silencing of OsDGKs on OsWRKY71 and OsNPR1 transcription induced by xylanase in rice protoplasts. After transient RNAi using two in vitro-synthesized dsRNAs against conserved regions of OsDGK(1, 2, 3, 7) and OsDGK(4, 5, 8) for 24 h, the transfected protoplasts were treated with 150 μg/ml xylanase for 6 h. The protoplasts were collected for real-time PCR analysis to detect transcripts of OsWRKY71 (A) and OsNPR1 (B). Values followed by different letters differ significantly (P < 0.01).