Table A4.
List of oligos used in amplifying DNA templates for in vitro dsRNA synthesis.
| Targeted Gene | Directions | Oligos (5′–3′) | Size of dsRNA (bp) |
|---|---|---|---|
| OsPLDα1 | Forward | GCTTAATACGACTCACTATAGGGAGGTTGACGATGAGTACATCATCATCGG | 486 |
| Reverse | GCTTAATACGACTCACTATAGGGAGGCTATGAGGTGAGGATGGGGGGCATG | ||
| OsDGKs(1,2,3,7) | Forward | GCTTAATACGACTCACTATAGGGAGGTGTCGGCTTTCGCGATGCCT | 208 |
| Reverse | GCTTAATACGACTCACTATAGGGAGGGGCTGCTTCCATGGCTCCCC | ||
| OsDGKs(4,5,8) | Forward | GCTTAATACGACTCACTATAGGGAGGCGCGCGCAGAGGTTAGCTCA | 229 |
| Reverse | GCTTAATACGACTCACTATAGGGAGGGGAGGCCGCATGGCCGATAG | ||
| OsDGK2 (3′-UTR) | Forward | GCTTAATACGACTCACTATAGGGAGGAATTGGTATCTTTTCTAGGTTGCAT | 260 |
| Reverse | GCTTAATACGACTCACTATAGGGAGGTCTGCTGAACAATAAACAAGAAATC | ||
| OsDGK3 (3′-UTR) | Forward | GCTTAATACGACTCACTATAGGGAGGAATCAGGGTCGTATTCTAGATCGTT | 303 |
| Reverse | GCTTAATACGACTCACTATAGGGAGGCATAACAGGAGGGGAAATCTGAGTA | ||
| OsDGK7 (3′-UTR) | Forward | GCTTAATACGACTCACTATAGGGAGGTGACGAGGTTTTGTACGTATGGCTG | 342 |
| Reverse | GCTTAATACGACTCACTATAGGGAGGCGTGGAGGTATATTCTGCGGGTAGT |