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. 2012 Mar 28;3:53. doi: 10.3389/fpls.2012.00053

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Copyright © 2012 Benning, Tamot, Guelette and Hoffmann-Benning.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Lipid-binding assays. (A) Protein–lipid overlay assay using purified PLAFP. Ten nanomole of selected phospholipids were spotted on a hybond-C membrane and overlaid it with 1 μl/ml purified PLAFP. PE, phosphatidyl ethanolamine; PA, phosphatidic acid; PC, phosphatidyl choline; PS, phosphatidyl serine; PG, phosphatidyl glycerol; PI, phosphatidyl inositol. (B) Liposome binding assay using purified PLAFP. Liposomes were prepared from PC, PA, or PC:PA (1:1, w/w). Bound protein was detected by antibody against the His-tag.