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Purification of PLAFP. (Top) SDS-PAGE gel for different fractions of purification of PLAFP-His. M, molecular weight marker; CL, cleared lysate; FT, flow-through; W1, wash fraction; E1–E6, elution fractions. (Bottom) Western blot analysis for the above listed factions using Anti-His antibody to visualize PLAFP-His.
