Table 4.
Year | Variable | Time (days after sowing) | Case 1 | Case 2 | ||
---|---|---|---|---|---|---|
Maternal CO2 | Maternal CO2 | Plant type | Maternal CO2 × Plant type | |||
2005 | Disease severity | 76 | 0.606z | 0.780 | 0.005 | 0.759 |
2006 | Disease onset | 0.560 | 0.897 | 0.067 | 0.714 | |
Disease severity | 59 | 0.008 | 0.220 | <0.001 | 0.304 | |
69 | 0.020 | 0.328 | 0.001 | 0.095 | ||
AUDPC | 0.007 | 0.411 | <0.001 | 0.008 | ||
2007 | Disease severity | 55 | 0.631 | |||
63 | 0.413 | 0.755 | 0.001 | 0.081 | ||
69 | 0.365 | 0.621 | 0.004 | 0.147 | ||
76 | 0.514 | |||||
AUDPC | 0.457 | 0.894 | 0.001 | 0.201 |
Mixed models consisted of one-way ANOVA (Case 1) or two-way ANOVA (Case 2), and only fixed effects are presented (for random effects, see Materials and Methods). A more complex model with time as additional factor resulted in interactions between time and other factors (data not shown). P values ≤ 0.05 were indicated in bold digits, P values ≤ 0.1 and > 0.05 were underlined. Empty fields and missing time points represent variables which failed to meet the assumptions of ANOVA. zIncluding only individuals with known plant type in Case 1. If individuals of unknown plant type (flower color was not determined) were also included, the ANOVA of disease severity resulted in P < 0.001. In the latter case, disease severity was significantly higher for high-CO2 (440 and 600 ppm maternal CO2) than for low-CO2 progenies (280 ppm maternal CO2; Tukey–Kramer HSD test, P ≤ 0.05).