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. 2012 Jan 16;2:118. doi: 10.3389/fpls.2011.00118

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Copyright © 2012 Machettira, Groß, Tillmann, Weis, Englich, Sommer, Königer and Schleiff.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Co-expression of outer and inner chloroplast membrane proteins at high levels induces many types of membrane alterations including stromule-like structures. A. thaliana mesophyll protoplasts were co-transfected with atTic20-II–GFP (inner envelope) and TM-CHUP1–Cherry (outer envelope; see Figure 5E). GFP fluorescence (GFP), Cherry fluorescence (Cherry), chlorophyll autofluorescence (AUF) or overlays of GFP/AUF and GFP/Cherry of a representative protoplast are shown. Images are maximum projections of optical sections. The scale bar represents 10 μm. The framed region in the GFP/Cherry overlay is zoomed in bottom right (scale bare 2 μm). The triangles point to labeling with GFP or Cherry only. Scale bars represent 10 μm.