Table 1.
Selectivity filters and NPA boxes of S. moellendorffii MIPs.
| Subfamily | Proteina | ar/R selectivity filterb |
NPA motifsc |
||||
|---|---|---|---|---|---|---|---|
| H2 | H5 | LE1 | LE2 | Loop B | Loop E | ||
| PIP | SmPIP1;1 | F | H | T | R | NPA | NPA |
| SmPIP2;1 | F | H | S | R | NPA | NPA | |
| SmPIP2;2 | F | H | T | R | NPA | NPA | |
| PpPIP1;1 | F | H | T | R | NPA | NPA | |
| TIP | SmTIP6;1 | H | I | G | R | NPA | NPA |
| SmTIP6;2 | H | I | G | R | NPA | NPA | |
| PpTIP6;1 | H | I | A | R | NPA | NPG | |
| NIP | SmNIP3;1 | S | I | A | R | NPS | NPV |
| SmNIP3;2 | P | N | A | R | NPS | NPA | |
| SmNIP3;3 | A | N | A | R | NPA | NPC | |
| SmNIP3;4 | A | N | A | R | NPA | NPI | |
| SmNIP3;5 | S | I | A | R | NPA | NPV | |
| PpNIP3;1 | A | I | A | R | NPA | NPV | |
| SmNIP5;1 | F | A | A | R | NPA | NPA | |
| SmNIP5;2 | N | N | A | R | NPA | NPA | |
| PpNIP5;1 | F | A | A | R | NPA | NPA | |
| SmNIP7;1 | A | V | G | R | NPS | NPA | |
| PpNIP6;1 | G | V | A | R | NPA | NPM | |
| SIP | SmSIP1;1 | I | I | P | N | NPT | NPA |
| PpSIP1;1 | V | V | P | N | NPT | NPA | |
| XIP | SmXIP1;1 | H | V | A | R | NPC | NPA |
| SmXIP1;2 | H | I | A | R | NPC | NPA | |
| SmXIP1;3 | L | T | A | R | NPI | NPA | |
| PpXIP1;1 | Q | I | A | R | NPC | NPG | |
| HIP | SmHIP1;1 | H | H | A | R | NPA | NPA |
| SmHIP1;2 | H | H | A | R | NPA | NPA | |
| PpHIP1;1 | H | H | A | R | NPA | NPA | |
aMIPs from P. patens are included as a reference and are written in bold.
bThe ar/R filter is defined by one amino acid residue in helix 2, one in helix 5, and two in loop E. See Figure A2 in Appendix.
cThe sequences corresponding to the NPA motifs in loops B and E.