Fig. 5.

The LHX3 T194R protein is unable to activate pituitary target genes. a Expression vectors for WT and T194R LHX3 proteins were transiently cotransfected into mouse pituitary GHFT1 cells with a luciferase reporter gene under the control of theαGSU promoter. Promoter activity was assayed by measuring luciferase activity 48 h after transfection. Negative controls (Control) received equivalent amounts of empty expression vector plasmid. Activities are mean (light units/10 s/μg total protein) of triplicate assays ± SEM. A representative experiment of at least three experiments is depicted. b Similar experiments to those depicted in a were performed using a prolactin (PRL) promoter/enhancer reporter gene.