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Sulfation of trehalose by Stf0 is the first committed step in SL-1 biosynthesis. (A) The sulfotransferase Stf0 sulfates free trehalose to initiate SL-1 biosynthesis. (B) SL-1 is absent in the Δstf0 strain. Surface exposed lipids were removed by hexanes extraction of WT, Δstf0, and complemented (Δstf0 + pstf0) Mtb strains and analyzed by FT-ICR MS. SL-1 is observed as a collection of lipoforms that vary in the lengths of their acyl groups (±14 mass units).
