Table 1. Δstf0 Does Not Exhibit Global Defects in Cell Wall Integritya.
WT | Δstf0 | Δstf0 + pstf0 | ||
---|---|---|---|---|
detergents (% w/v) | SDS | 0.02 | 0.02 | 0.02 |
DOC | 0.010 | 0.007 | 0.008 | |
antibiotics (μg/mL) | rifampicin | 0.025 | 0.0125 | 0.0125 |
isoniazid | 0.025 | 0.027 | 0.030 | |
amikacin | 0.30 | 0.30 | 0.40 | |
kanamycin | 1.1 | 1.5 | >7.5b |
Mtb was grown in the presence of each detergent or antibiotic. The minimal inhibitory concentration that inhibited 50% of Mtb growth was noted after 7 days. These data are representative of at least two independent experiments each performed in duplicate.
Δstf0 + pstf0 contains a kanamycin resistance gene.