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RT-PCR demonstrating multiple TCUP transcripts present in azadC treated callus. Six separate transcripts are detected with a primer pair designed to amplify a 2.15-kb internal region of the TCUP coding sequence. Actin is shown as a control. Abbreviations: A1–A3, independent 25 μM azadC treated Hi-II A × B tissue culture cell lines; C1–C3, independent untreated Hi-II A × B tissue culture cell lines; P, Hi-II A × B seedling; B, BMS callus.
