Figure A1.

DNA gel blot comparing DNA methylation at the 5S ribosomal DNA loci in untreated and 5-aza-2-deoxycytidine treated Hi-II A × B tissue-cultured cell lines. The plant and callus DNA was digested with HpaII to examine and CpG methylation respectively. An increase in number and intensity of low molecular weight bands in the HpaII digested DNA indicates a decrease in DNA methylation. Hi-II A × B genomic DNA isolated from 14-day-old seedlings (S), from independent untreated control cultures (C1–C3), from independent 5-aza-2-deoxycytidine cultures (A1–A3).