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. 2011 Oct 21;2:67. doi: 10.3389/fpls.2011.00067

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Copyright © 2011 Narayanan, Ihemere, Chiu, Siritunga, Rajamani, Singh, Oda and Sayre.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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FEA1 does not transport cadmium in transgenic yeast. (A) RT-PCR analysis of FEA1 expression in Δfet3fet4 mutant yeast cells transformed with pYES2:FEA1. Actin expression was used as an internal control. (B) Growth rates of wild-type, iron uptake mutants (Δfet3fet4), and mutants complemented with pYES2:FEA1. Cell were grown in SC-URA medium containing 2% (w/v) galactose with various amounts (μM) of cadmium and iron (C) ICP-MS analysis of iron concentrations normalized for cell number. (D) ICP analysis of cadmium concentrations normalized for cell number. Data are means + SE of three replicate experiments.