Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
FEA1 protein facilitates uptake of ferrous iron in yeast. The yeast ferric reductase (Δfer1fer2) mutant was transformed with the pESC–LEU–FEA1 vector. (A) RT-PCR expression of FEA1 transgenic yeast. Actin was used as an internal expression control. (B) Growth of yeast on synthetic defined SG-LEU media containing 50 μM FeCl3 or SG-LEU media containing 50 μM FeCl3 + 1 mM sodium ascorbate. Plates were grown at 28°C for 2–3 days.
