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. 2012 Jan 9;2:114. doi: 10.3389/fpls.2011.00114

Figure 1.

Figure 1

Phylogenetic tree of plant PI4P 5-kinases and effects of PI4P 5-kinase overexpression on the morphology of tobacco pollen tubes. (A) Maximum likelihood (ML) tree created with the full-length protein sequences of A. thaliana PI4P 5-kinases and N. tabacum PIP6K6-1. Values on nodes represent% ML bootstrap support. Association of isoforms with PI4P 5-kinase subfamilies A and B according to Mueller-Roeber and Pical (2002), as indicated. (B–F) Selected PI4P 5-kinases of subfamily B were overexpressed in tobacco pollen tubes and the effects on growth were monitored. (B,C) Pollen tube branching (B) and protoplast trapping (C) upon expression of AtPIP5K5; (D, E) Pollen tube branching (D) and protoplast trapping (E) upon expression of NtPIP5K6-1; (F) Tip swelling upon expression of AtPIP5K2. Images are representative for ≥300 transgenic pollen tubes observed. Bars, 50 μm.