Figure 9.

Rate of evolution of Arabidospis thaliana PI4P 5-kinase sequences according to sliding window analysis of K_A/K_S ratios comparing orthologous sequences of A. thaliana and A. lyrata. The rate of sequence evolution across the coding sequence of AtPIP5K1-9 was assessed by comparing the A. thaliana sequences to orthologous sequences from A. lyrata. The sliding window analysis depicts the ratios of the number of non-synonymous substitutions per non-synonymous site (K_A) to the number of synonymous substitutions per synonymous site (K_S). All plots are drawn to the same scale. Increased K_A/K_S ratios in sequence regions of AtPIP5K2 encoding the NT-domain, the Lin-domain and the variable insert region are annotated in the AtPIP5K2 plot, as indicated. The pairs of orthologous genes from A. thaliana and A. lyrata used for the analysis are given in the Section “Materials and Methods.”