Figure 5.

Subcellular localization of Vps25 and Vps36. BY-2 cells were co-transformed with the indicated gene constructs and then cells were processed, imaged, and analyzed either by epifluorescence microscopy [(A,B) top row] or [(B) bottom row] CLSM as described in the legend of Figure 2. The yellow color in the corresponding merged images indicates co-localization between co-expressed proteins; white arrowheads also indicate obvious protein co-localization, while open arrowheads in (A) indicate the localization of HA-Vps36 (but not co-expressed GFP-Syp21) at the plasma membrane. Bar = 10 μm.