Table 1.
Endosomal sorting complex required for transport and ESCRT-associated proteins in yeasts, mammals, and Arabidopsis.
| Complex | Yeasta | Mammalsa | Arabidopsisb | |
|---|---|---|---|---|
| Proteinc | AGId | |||
| ESCRT-0 | Vps27 | HRS | ||
| Hse1 | STAM1 | |||
| STAM2 | ||||
| Other proteins known or proposed to be involved in ubiquitinated cargo protein-sorting and/or interaction with ESCRT-I | Tom1L1 | Tom1A | At2g38410 | |
| Tom1L2-1 | Tom1B | At5g01760 | ||
| Tom1L2-3 | Tom1C | At1g76970 | ||
| Tom1L3 | Tom1D | At4g32760 | ||
| Tom1 | Tom1E | At1g06210 | ||
| Tom1F | At1g21380 | |||
| Tom_L | At5g16880 | |||
| Tom1G | At5g63640 | |||
| Tom1H | At3g08790 | |||
| ESCRT-I | Vps23 | VPS23/TSG101 | Vps23A (ELC) | At3g12400 |
| Vps23B (ELC-like) | At5g13860 | |||
| Vps28 | VPS28 | Vps28A | At4g05000 | |
| Vps28B | At4g21560 | |||
| Vps37 | VPS37A | Vps37A | At3g53120 | |
| VPS37B | Vps37B | At2g36680 | ||
| VPS37C | ||||
| VPS37D | ||||
| Mvb12 | MVB12 | |||
| ESCRT-II | Vps22 | EAP30 | Vps22 | At4g27040 |
| Vps25 | EAP20 | Vps25 | At4g19003 | |
| Vps36 | EAP45 | Vps36 | At5g04920 | |
| ESCRT-III | Vps2/Did4 | CHMP2A | Chmp2A | At2g06530 |
| CHMP2B | Chmp2B | At5g44560 | ||
| Chmp2C | At1g03950 | |||
| Vps20 | CHMP6 | Vps20A | At5g09260 | |
| Vps20B | At5g63880 | |||
| Vps24 | CHMP3 | Vps24A | At5g22950 | |
| Vps24B | At3g45000 | |||
| Snf7/Vps32 | CHMP4A | Snf7A (Vps32.1) | At2g19830 | |
| CHMP4B | Snf7B (Vps32.2) | At4g29160 | ||
| CHMP4C | ||||
| Vps4 and associated regulators | Vps4 | VPS4A | Vps4 (SKD1) | At2g27600 |
| VPS4B | ||||
| Vps60 | CHMP5 | Vps60A | At3g10640 | |
| Vps60B | At5g04850 | |||
| Did2/Vps46 | CHMP1A | Chmp1A | At1g73030 | |
| CHMP1B | Chmp1B | At1g17730 | ||
| Bro1/Vps31 | ALIX/AIP1 | Bro1 | At1g15130 | |
| Vta1 | VTA1/LIP5 | Vta1 (LIP5) | At4g26750 | |
aNomenclature of yeast (S. cerevisiae) and mammalian (human) ESCRT and ESCRT-associated proteins is based on those previously published (Hurley and Hanson, 2010), including for the Arabidopsis Tom1 proteins (Winter and Hauser, 2006). bArabidopsis ESCRT and ESCRT-associated proteins were identified previously using BLASTp analyses with amino acid sequences of known class E Vps proteins from yeasts and humans as queries (Mullen et al., 2006; Spitzer et al., 2006; Winter and Hauser, 2006), or using comparative genomic analyses of coding sequences (Leung et al., 2008). cNomenclature of Arabidopsis ESCRT and ESCRT-associated proteins used in this study. Alternate nomenclature of selected Arabidopsis proteins indicated in brackets are based on Spitzer et al. (2006), Haas et al. (2007) and Shahriari et al. (2010). dArabidopsis gene identifier (AGI) number represents the systematic designation given to each locus, gene, and its corresponding protein product(s) by the Arabidopsis Information Resource (http://www.arabidopsis.org). Alix, ALG-2-interacting protein x; Aip1, apoptosis-linked gene (ALG)-2-interacting protein; Bro1, bypass requirement for protein kinase C homolog (BCK) 1-like resistance to osmotic shock; Chmp, charged multivesicular body protein; Did2, DOA4-independent degradation 2; EAP, RNA polymerase II elongation factor ELL-associated protein; ELC, elch (German for elk); HRS, hepatocyte growth factor-regulated tyrosine kinase substrate; LIP5, lysosomal trafficking regulator (lyst) interacting protein 5; Snf7, sucrose non-fermenting 7; STAM, signal-transducing adaptor molecule; TSG101, tumor susceptibility gene 101; SKD1, suppressor of K+ transport growth defect 1; HseI, Hbp, STAM, and EAST; Tom1, target of myb 1; Vta1, Vps20-associated 1.