Table 1.
Candidate target proteins identified using the yeast-based screening platform for profiling of small molecule interactions.
| Protein targeta | Function | Locus ID | Bait | cDNA Library | Activation of lacZb | Competed out by Mtxb |
|---|---|---|---|---|---|---|
| EH (7×) | Epoxide hydrolase | At3g51000 | Mtx–CAMe | Wounded leaves | Yesc | Yes |
| MTK (1×) | 5-Methylthioribose kinase | At1g49820 | Mtx–CAMe | Wounded leaves | Noc | Yes |
| PP2C6d (1×) | Serine/threonine protein phosphatase C2 | At3g55050 | Mtx–SA | Wounded leaves | No | Yes |
| SPL3 (37×) | Transcription factor | At2g33810 | Mtx–Cpd8 | Inflorescence | nde | Yes |
| SPL4 (2×) | Transcription factor | At1g53160 | Mtx–Cpd8 | Inflorescence | nd | Yes |
| SPL13 (1×) | Transcription factor | At5g50570 | Mtx–Cpd8 | Wounded leaves | nd | Yes |
Candidate targets were identified by screening for yeast growth on selective medium (–Leu) supplemented with the bait indicated. A total of about 106 colonies were screened for each library and bait. No candidates were obtained with Mtx–JA, Mtx–CA, and Mtx–ABA.
aThe number of hits in brackets shown for each target represents the total number of independent yeast colonies identified during the cDNA library screenings.
bAll initial candidates were validated by retransfection into new yeast cells, activation of the second reporter gene (lacZ), and competition between the bait and Mtx.
cSee Figure 4B.
dThe corresponding cDNA is not full length, the protein is truncated at the N-terminus.
eNot determined.